Reducing Electron Beam Damage with Multipass Transmission Electron Microscopy

Reducing Electron Beam Damage with Multipass Transmission Electron Microscopy

With the introduction of hardware aberration correction, direct electron detectors, ultra-bright electron sources and highly precise spectrometers, it seems like we are approaching the pinnacle of transmission electron microscopy (TEM) imaging and spectroscopy. However, the field of electron microscopy is still far from the ultimate signal-to-noise and efficiency limits imposed by electron scat...

Diagnostic Transmission Electron Microscopy

The high resolution and sensitivity of electron microscopy is still a valuable tool, and for some diseases it is the gold standard in pathological diagnosis. Today the sample turnaround time for processing in the lab can be significantly reduced from days to hours by the microwave technology. In urgent clinical cases, microwaveassisted tissue processing, in combination with digital image acquis...

Electron Diffraction Using Transmission Electron Microscopy

Electron diffraction via the transmission electron microscope is a powerful method for characterizing the structure of materials, including perfect crystals and defect structures. The advantages of electron diffraction over other methods, e.g., x-ray or neutron, arise from the extremely short wavelength (≈2 pm), the strong atomic scattering, and the ability to examine tiny volumes of matter (≈1...

Conventional transmission electron microscopy

Researchers have used transmission electron microscopy (TEM) to make contributions to cell biology for well over 50 years, and TEM continues to be an important technology in our field. We briefly present for the neophyte the components of a TEM-based study, beginning with sample preparation through imaging of the samples. We point out the limitations of TEM and issues to be considered during ex...

Electron Microscopy of Fowl Pox Virus